Hotspot

trnL-CAA-ndhB

IRb · 95235-95863 · 629 bp

Back to Medinilla

Pi 0.00993

MarkerSeek score 69.80

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 629
variable_sites: 13
pi: 0.00993
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	664-715 bp	1.0	68.8
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	662-713 bp	1.0	68.8
trnL-CAA-ndhB_p3	GTCTCGCGTGTCTACCATTT	TCAATTGCTCAGGATACCCT	50.0	45.0	58.0	55.9	637-688 bp	1.0	68.7
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	TCAATTGCTCAGGATACCCT	50.0	45.0	58.0	55.9	635-686 bp	1.0	68.7
trnL-CAA-ndhB_p5	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.1	57.9	652-703 bp	1.0	68.2