Hotspot

rps12-clpP

LSC · 70751-70937 · 187 bp

Back to Medinilla

Pi 0.0321

MarkerSeek score 69.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 13
pi: 0.0321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GGATTGGCTGTCTTGTGTTTC	TTATGTCAGCAACGGAAGCC	47.6	50.0	57.7	58.6	272-292 bp	1.0	72.9
rps12-clpP_p2	GGATTGGCTGTCTTGTGTTT	TTATGTCAGCAACGGAAGCC	45.0	50.0	56.5	58.6	272-292 bp	1.0	71.4
rps12-clpP_p3	GGATTGGCTGTCTTGTGTTTC	GCCCAAGCTCATGGAATTGT	47.6	50.0	57.7	58.8	255-275 bp	1.0	71.0
rps12-clpP_p4	GGATTGGCTGTCTTGTGTTT	GCCCAAGCTCATGGAATTGT	45.0	50.0	56.5	58.8	255-275 bp	1.0	69.6
rps12-clpP_p5	GGATTGGCTGTCTTGTGTTTC	TATGTCAGCAACGGAAGCC	47.6	52.6	57.7	57.8	271-291 bp	1.0	68.1