Hotspot

petA-psbJ

LSC · 63628-64548 · 921 bp

Back to Medinilla

Pi 0.0168

MarkerSeek score 61.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 32
pi: 0.0168
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTGGGTGGATTTGGTCAAG	GGCGGAGGAAGCAAGATAAA	50.0	50.0	58.0	58.0	1192-1226 bp	1.0	85.1
petA-psbJ_p2	GAGCTTCTCGTTTCAGAGGG	GGCGGAGGAAGCAAGATAAA	55.0	50.0	58.0	58.0	1254-1288 bp	1.0	84.8
petA-psbJ_p3	GAGCTTCTCGTTTCAGAGGG	CTTGATGAAATGGCGGAGGA	55.0	50.0	58.0	58.0	1265-1299 bp	1.0	84.8
petA-psbJ_p4	GAGCTTCTCGTTTCAGAGGG	TGATGAAATGGCGGAGGAAG	55.0	50.0	58.0	58.0	1263-1297 bp	1.0	84.8
petA-psbJ_p5	GAGCTTCTCGTTTCAGAGGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1507-1541 bp	1.0	84.5