Hotspot

ndhC-trnV-UAC

LSC · 51391-52102 · 712 bp

Back to Medinilla

Pi 0.0278
MarkerSeek score 71.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
712
variable_sites
38
pi
0.0278
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAAATGCCCAGAAAATATCA GGTTCGAATCCGTATAGCCC 33.3 55.0 53.1 57.9 749-818 bp 1.0 47.8
ndhC-trnV-UAC_p2 AGAAATGCCCAGAAAATATCA CGGTTCGAATCCGTATAGCC 33.3 55.0 53.1 58.3 750-819 bp 1.0 47.8
ndhC-trnV-UAC_p3 AGAAATGCCCAGAAAATATCAT GGTTCGAATCCGTATAGCCC 31.8 55.0 53.8 57.9 749-818 bp 1.0 47.8
ndhC-trnV-UAC_p4 AGAAATGCCCAGAAAATATCAT CGGTTCGAATCCGTATAGCC 31.8 55.0 53.8 58.3 750-819 bp 1.0 47.8
ndhC-trnV-UAC_p5 AGAAATGCCCAGAAAATATCA CGAAAGAGCCCAATGCAAAA 33.3 45.0 53.1 57.6 847-916 bp 1.0 47.4