Hotspot

trnT-GGU-psbD

LSC · 32273-33481 · 1209 bp

Back to Medinilla

Pi 0.0201

MarkerSeek score 65.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 50
pi: 0.0201
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AATTTACCAAGAGCTATAGTCA	55.0	31.8	58.1	52.3	1199-1296 bp	1.0	47.2
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	AATTTACCAAGAGCTATAGTCA	50.0	31.8	58.3	52.3	1176-1273 bp	1.0	47.2
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	AATTTACCAAGAGCTATAGTCA	50.0	31.8	58.1	52.3	1202-1299 bp	1.0	47.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AAATTTACCAAGAGCTATAGTCA	55.0	30.4	58.1	53.2	1200-1297 bp	1.0	47.1
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	AAATTTACCAAGAGCTATAGTCA	50.0	30.4	58.1	53.2	1203-1300 bp	1.0	47.1