Hotspot

trnE-UUC-trnT-GGU

LSC · 31318-32200 · 883 bp

Back to Medinilla

Pi 0.0247

MarkerSeek score 64.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 42
pi: 0.0247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGGATCAGGGAATTCTTGGG	CAAGACTACGGAATCGGCAA	55.0	50.0	58.0	58.0	1310-1638 bp	1.0	87.4
trnE-UUC-trnT-GGU_p2	CGGATCAGGGAATTCTTGGG	AACAAGACTACGGAATCGGC	55.0	50.0	58.0	58.0	1312-1640 bp	1.0	87.4
trnE-UUC-trnT-GGU_p3	TCATTTCATGGCGGATCAGG	CAAGACTACGGAATCGGCAA	50.0	50.0	58.0	58.0	1321-1649 bp	1.0	87.3
trnE-UUC-trnT-GGU_p4	CGGATCAGGGAATTCTTGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	930-1233 bp	1.0	87.2
trnE-UUC-trnT-GGU_p5	TCATTTCATGGCGGATCAGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	941-1244 bp	1.0	87.2