Hotspot

psbM-trnD-GUC

LSC · 29839-30572 · 734 bp

Back to Medinilla

Pi 0.0136

MarkerSeek score 71.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 21
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACTTTGACTCACGGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.4	58.6	852-918 bp	1.0	61.1
psbM-trnD-GUC_p2	TCACTTTGACTCACGGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.2	58.6	852-918 bp	1.0	59.4
psbM-trnD-GUC_p3	AATCACTTTGACTCACGGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	854-920 bp	1.0	59.2
psbM-trnD-GUC_p4	ATCACTTTGACTCACGGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	853-919 bp	1.0	59.2
psbM-trnD-GUC_p5	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	798-864 bp	1.0	59.1