Hotspot

trnR-UCU-atpA

LSC · 9766-10065 · 300 bp

Back to Medinilla

Pi 0.0339

MarkerSeek score 70.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 21
pi: 0.0339
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACAGATGGAACGTTTTGGACT	47.6	42.9	57.3	57.8	346-354 bp	1.0	65.6
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	ACAGATGGAACGTTTTGGAC	47.6	45.0	57.3	56.3	346-354 bp	1.0	61.4
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	ACAGATGGAACGTTTTGGACT	42.9	42.9	56.6	57.8	345-353 bp	1.0	60.0
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AACAGATGGAACGTTTTGGAC	47.6	42.9	57.3	57.0	347-355 bp	1.0	59.4
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CAGATGGAACGTTTTGGACT	47.6	45.0	57.3	56.0	345-353 bp	1.0	59.1