Hotspot

trnS-GCU-trnR-UCU

LSC · 7896-9693 · 1798 bp

Back to Medinilla

Pi 0.0207

MarkerSeek score 69.40

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1798
variable_sites: 75
pi: 0.0207
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1847-1912 bp	1.0	75.6
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1846-1911 bp	1.0	75.6
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1848-1913 bp	1.0	73.6
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1847-1912 bp	1.0	73.6
trnS-GCU-trnR-UCU_p5	AGCAATCCGACGCTTTAGTC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.4	56.6	1849-1914 bp	1.0	72.8