Hotspot

ycf2

Genome · 85814-91342 · 5529 bp

Back to Medicago

Pi 0.0338

MarkerSeek score 64.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5529
variable_sites: 885
pi: 0.0338
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AAGAGAATTGGCGGAATCCC	AATTCGCTTGGATCCATCCC	50.0	50.0	57.9	58.0	527-800 bp	0.619048	76.2
ycf2_p2	GAGAAGAAGAAGCGCAGGAA	AATTCGCTTGGATCCATCCC	50.0	50.0	57.9	58.0	588-861 bp	0.619048	76.0
ycf2_p3	AAGAGAAGAAGAAGCGCAGG	AATTCGCTTGGATCCATCCC	50.0	50.0	57.9	58.0	590-863 bp	0.619048	75.9
ycf2_p4	CGATCAAGAGAATTGGCGGA	AATTCGCTTGGATCCATCCC	50.0	50.0	58.1	58.0	562-805 bp	0.571429	72.1
ycf2_p5	ATATGCGGGAAGGTCCTGTA	AATTCGCTTGGATCCATCCC	50.0	50.0	57.9	58.0	433-484 bp	0.238095	60.6