Hotspot

accD

Genome · 55739-57928 · 2190 bp

Back to Medicago

Pi 0.118

MarkerSeek score 71.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 2190
variable_sites: 768
pi: 0.118
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCCCGCTCTCTTGTTCTAT	TTGCAACTGCCGGAAATACT	55.0	45.0	58.9	58.1	1852-2957 bp	0.714286	72.8
accD_p2	CCCCCGCTCTCTTGTTCTAT	AAAGAAGCCATTGCAACTGC	55.0	45.0	58.9	57.8	1862-2967 bp	0.714286	72.3
accD_p3	CCCCGCTCTCTTGTTCTATT	TTGCAACTGCCGGAAATACT	50.0	45.0	57.0	58.1	1851-2956 bp	0.714286	71.9
accD_p4	CCCCGCTCTCTTGTTCTATT	AAAGAAGCCATTGCAACTGC	50.0	45.0	57.0	57.8	1861-2966 bp	0.714286	71.4
accD_p5	AAAGGCGGGTGGTTTTATGT	AGGCCCACTAAAGGAACAAA	45.0	45.0	58.0	56.9	1951-2999 bp	0.571429	66.3