Hotspot

ycf1-rps15

Genome · 110870-111293 · 424 bp

Back to Medicago

Pi 0.0708

MarkerSeek score 77.60

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 424
variable_sites: 117
pi: 0.0708
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGTATCCTCGATTGAAAGATT	TCAACTGATCCGGGGTTTTC	33.3	50.0	52.4	57.8	326-650 bp	0.952381	50.1
ycf1-rps15_p2	TGTATCCTCGATTGAAAGATT	CAACTGATCCGGGGTTTTCT	33.3	50.0	52.4	57.8	325-649 bp	0.952381	50.1
ycf1-rps15_p3	ATGTATCCTCGATTGAAAGAT	TCAACTGATCCGGGGTTTTC	33.3	50.0	52.1	57.8	327-651 bp	0.952381	50.1
ycf1-rps15_p4	ATGTATCCTCGATTGAAAGAT	CAACTGATCCGGGGTTTTCT	33.3	50.0	52.1	57.8	326-650 bp	0.952381	50.1
ycf1-rps15_p5	TGTATCCTCGATTGAAAGATT	ACTGATCCGGGGTTTTCTTC	33.3	50.0	52.4	57.5	323-647 bp	0.952381	50.1