Hotspot

rrn16-trnI-GAU

Genome · 98587-98895 · 309 bp

Back to Medicago

Pi 0.0687

MarkerSeek score 85.20

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 309
variable_sites: 104
pi: 0.0687
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	402-621 bp	1.0	88.1
rrn16-trnI-GAU_p2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	424-643 bp	1.0	88.0
rrn16-trnI-GAU_p3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	50.0	50.0	57.6	58.2	411-630 bp	1.0	87.6
rrn16-trnI-GAU_p4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	404-623 bp	1.0	87.6
rrn16-trnI-GAU_p5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	403-622 bp	1.0	87.6