Hotspot

rpl23-trnI-CAU

Genome · 84996-85626 · 631 bp

Back to Medicago

Pi 0.115

MarkerSeek score 76.80

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: Genome
length_bp: 631
variable_sites: 202
pi: 0.115
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	CACTTGACTGTCTACGGCTC	ATTGGCGAAGTCGTAGGTTC	55.0	50.0	58.0	58.0	774-1203 bp	0.857143	86.1
rpl23-trnI-CAU_p2	CACTTGACTGTCTACGGCTC	GGCGAAGTCGTAGGTTCAAT	55.0	50.0	58.0	58.0	771-1200 bp	0.857143	86.1
rpl23-trnI-CAU_p3	CACTTGACTGTCTACGGCTC	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	799-1228 bp	0.857143	86.1
rpl23-trnI-CAU_p4	CACTTGACTGTCTACGGCTC	TATCGATTGCCTTTGCGTGA	55.0	45.0	58.0	58.0	1112-1553 bp	0.761905	82.2
rpl23-trnI-CAU_p5	CACTTGACTGTCTACGGCTC	TATCATTGCTGGGTCCAACG	55.0	50.0	58.0	58.0	1255-1696 bp	0.571429	72.6