Hotspot

rps12-clpP

Genome · 67986-68854 · 869 bp

Back to Medicago

Pi 0.162

MarkerSeek score 76.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 869
variable_sites: 475
pi: 0.162
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GATTCGGTTTGCACCAATCG	AAGCCCACGGAATTGTTGAT	50.0	45.0	58.1	58.1	680-2454 bp	0.809524	82.9
rps12-clpP_p2	TCCAATGGCTTTTGCTGCTA	AAGCCCACGGAATTGTTGAT	45.0	45.0	58.1	58.1	1164-2946 bp	0.714286	79.3
rps12-clpP_p3	ATTCCAATGGCTTTTGCTGC	AAGCCCACGGAATTGTTGAT	45.0	45.0	57.9	58.1	1166-2948 bp	0.714286	79.1
rps12-clpP_p4	TCCAATGGCTTTTGCTGCTA	CTTTTCATGAGGGACGAGCA	45.0	50.0	58.1	57.9	1787 bp	0.047619	40.5
rps12-clpP_p5	TCCAATGGCTTTTGCTGCTA	TTTTCATGAGGGACGAGCAG	45.0	50.0	58.1	57.9	1786 bp	0.047619	40.5