Hotspot

trnQ-UUG-accD

Genome · 54545-55738 · 1194 bp

Back to Medicago

Pi 0.0794

MarkerSeek score 75.90

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 1194
variable_sites: 499
pi: 0.0794
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	AATGGGATCCACAGAGACCA	55.0	50.0	58.1	58.0	1160-2707 bp	0.857143	85.4
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	ATGGGATCCACAGAGACCAT	55.0	50.0	58.1	57.8	1159-2706 bp	0.857143	84.1
trnQ-UUG-accD_p3	TGGAATTGACGAACAACCCA	AATGGGATCCACAGAGACCA	45.0	50.0	57.6	58.0	1258-2805 bp	0.809524	81.3
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	CAATGGGATCCACAGAGACC	55.0	55.0	58.1	57.7	1161-2708 bp	0.809524	81.1
trnQ-UUG-accD_p5	GAGGTTCGAATCCTTCCGTC	CCATAACAATTGTCGCAGGC	55.0	50.0	58.1	57.8	1745-2457 bp	0.761905	80.3