Hotspot

trnG-GCC-psbZ

Genome · 24408-25020 · 613 bp

Back to Medicago

Pi 0.0568

MarkerSeek score 81.20

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: Genome
length_bp: 613
variable_sites: 140
pi: 0.0568
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGGATAGTGGGAATCGAACC	TTGCTTCTCCTGATGGTTGG	55.0	50.0	57.8	57.8	623-883 bp	1.0	89.2
trnG-GCC-psbZ_p2	CGGATAGTGGGAATCGAACC	GCTTCTCCTGATGGTTGGTC	55.0	55.0	57.8	58.3	621-881 bp	1.0	88.7
trnG-GCC-psbZ_p3	GCGGATAGTGGGAATCGAAC	TTGCTTCTCCTGATGGTTGG	55.0	50.0	58.5	57.8	624-884 bp	1.0	86.4
trnG-GCC-psbZ_p4	GGATAGTGGGAATCGAACCC	TTGCTTCTCCTGATGGTTGG	55.0	50.0	57.5	57.8	622-882 bp	1.0	86.0
trnG-GCC-psbZ_p5	GCGGATAGTGGGAATCGAAC	GCTTCTCCTGATGGTTGGTC	55.0	55.0	58.5	58.3	622-882 bp	1.0	85.9