Hotspot

trnH-GUG-psbA

Genome · 78-490 · 413 bp

Back to Medicago

Pi 0.0317

MarkerSeek score 75.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 413
variable_sites: 85
pi: 0.0317
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	313-512 bp	0.904762	86.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	327-526 bp	0.904762	86.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	326-525 bp	0.904762	86.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	328-527 bp	0.904762	86.5
trnH-GUG-psbA_p5	GCGAATGACGGGAATTGAAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	365-564 bp	0.904762	86.2