Hotspot

ndhF

SSC · 109435-111654 · 2220 bp

Back to Meconopsis

Pi 0.0213

MarkerSeek score 62.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 187
pi: 0.0213
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	TGGGGGTCAAGCTTTTGATC	50.0	50.0	58.0	58.1	2863-2905 bp	0.666667	75.2
ndhF_p2	CTCAAAGAAACCGCCGAAAC	TGGGGGTCAAGCTTTTGATC	50.0	50.0	58.0	58.1	2941-2983 bp	0.666667	75.0
ndhF_p3	GAAACTCAAAGAAACCGCCG	TGGGGGTCAAGCTTTTGATC	50.0	50.0	58.0	58.1	2945-2987 bp	0.666667	75.0
ndhF_p4	CCTTCCCCTTCCCTTTGTTC	TGGGGGTCAAGCTTTTGATC	55.0	50.0	58.1	58.1	2818-2860 bp	0.6	70.9
ndhF_p5	CCTTCCCCTTCCCTTTGTTC	AGTCGTGGGTATCAAAGTGC	55.0	50.0	58.1	57.9	2950-2992 bp	0.533333	67.3