Hotspot

rpl32-trnL-UAG

SSC · 112792-113404 · 613 bp

Back to Meconopsis

Pi 0.0444

MarkerSeek score 80.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 613
variable_sites: 108
pi: 0.0444
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTGCGCTAAAAGCCTTTTCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	514-750 bp	1.0	91.8
rpl32-trnL-UAG_p2	CTGCGCTAAAAGCCTTTTCC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	515-751 bp	1.0	91.8
rpl32-trnL-UAG_p3	CTGCGCTAAAAGCCTTTTCC	TTTAGAAGATGCCGCCACTC	50.0	50.0	58.0	58.0	574-810 bp	1.0	91.7
rpl32-trnL-UAG_p4	CTGCGCTAAAAGCCTTTTCC	GGAAAAGCCCCATGAAAGGA	50.0	50.0	58.0	58.1	914-1164 bp	1.0	91.4
rpl32-trnL-UAG_p5	GCACTTTGATACCCACGACT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1285-1432 bp	0.666667	77.8