Hotspot

ndhF-rpl32

SSC · 111655-112617 · 963 bp

Back to Meconopsis

Pi 0.0567

MarkerSeek score 85.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 963
variable_sites: 206
pi: 0.0567
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCATATTGATATGTATGTTCCA	GTACGTTTCTTTGGAACGGC	30.4	50.0	53.7	57.7	894-1179 bp	0.933333	49.3
ndhF-rpl32_p2	TGCATATTGATATGTATGTTCCA	GGAAAAGGCTTTTAGCGCAG	30.4	50.0	53.7	58.0	970-1255 bp	0.866667	46.7
ndhF-rpl32_p3	TGCATATTGATATGTATGTTCCA	AGGAAAAGGCTTTTAGCGCA	30.4	45.0	53.7	58.4	971-1256 bp	0.866667	46.7
ndhF-rpl32_p4	ATGCATATTGATATGTATGTTCCA	GGAAAAGGCTTTTAGCGCAG	29.2	50.0	54.3	58.0	971-1256 bp	0.866667	46.7
ndhF-rpl32_p5	TGCATATTGATATGTATGTTCCAT	GGAAAAGGCTTTTAGCGCAG	29.2	50.0	54.3	58.0	970-1255 bp	0.866667	46.7