Hotspot

trnF-GAA-ndhJ

LSC · 47530-48278 · 749 bp

Back to Meconopsis

Pi 0.0345

MarkerSeek score 83.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 116
pi: 0.0345
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AGGGTTGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	871-909 bp	1.0	91.1
trnF-GAA-ndhJ_p2	GGGTTGGGATAGCTCAGTTG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	870-908 bp	1.0	90.9
trnF-GAA-ndhJ_p3	GGTTGGGATAGCTCAGTTGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	869-907 bp	1.0	90.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	829-867 bp	1.0	90.9
trnF-GAA-ndhJ_p5	AAGGGTTGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	872-910 bp	0.933333	88.4