Hotspot

atpH-atpI

LSC · 13619-14563 · 945 bp

Back to Meconopsis

Pi 0.0246

MarkerSeek score 79.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 112
pi: 0.0246
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1002-1032 bp	1.0	87.6
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	CGAATCCATGGAGGGTCATC	50.0	55.0	58.1	57.8	1090-1120 bp	1.0	87.3
atpH-atpI_p3	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	1061-1091 bp	1.0	87.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1003-1033 bp	1.0	86.5
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1062-1092 bp	1.0	86.3