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trnK-UUU-rps16

LSC · 4164-4752 · 589 bp

Back to Meconopsis

Pi 0.0426

MarkerSeek score 82.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 102
pi: 0.0426
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	CAAAAGGTATGTTGCTGCCG	55.0	50.0	58.0	58.0	1371-1526 bp	0.933333	89.1
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CAAAAGGTATGTTGCTGCCG	55.0	50.0	58.0	58.0	1370-1525 bp	0.933333	89.1
trnK-UUU-rps16_p3	CTCGACCCATCCGTGAATTT	CAAAAGGTATGTTGCTGCCG	50.0	50.0	58.0	58.0	1498-1653 bp	0.933333	89.0
trnK-UUU-rps16_p4	TCGACCCATCCGTGAATTTC	CAAAAGGTATGTTGCTGCCG	50.0	50.0	58.0	58.0	1497-1652 bp	0.8	83.7
trnK-UUU-rps16_p5	CGACCCATCCGTGAATTTCT	CAAAAGGTATGTTGCTGCCG	50.0	50.0	58.0	58.0	1496-1651 bp	0.8	83.6