Hotspot

ycf80

Genome · 57876-59282 · 1407 bp

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Pi 0.0839

MarkerSeek score 77.60

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1407
variable_sites: 118
pi: 0.0839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	ACTAGGCATTGCACCAACAA	AAACAAGGAGGTTGCTCTGG	45.0	50.0	58.0	58.0	2161-2177 bp	1.0	85.6
ycf80_p2	TTGCACCAACAATACCAGCT	AAACAAGGAGGTTGCTCTGG	45.0	50.0	58.0	58.0	2153-2169 bp	1.0	85.6
ycf80_p3	GGCATTGCACCAACAATACC	AAACAAGGAGGTTGCTCTGG	50.0	50.0	58.0	58.0	2157-2173 bp	1.0	85.6
ycf80_p4	ACCAGCTGCTCGACAATTAG	AAACAAGGAGGTTGCTCTGG	50.0	50.0	58.0	58.0	2140-2156 bp	1.0	85.5
ycf80_p5	CCAGCTGCTCGACAATTAGT	AAACAAGGAGGTTGCTCTGG	50.0	50.0	58.0	58.0	2139-2155 bp	1.0	85.5