Hotspot

trnS(gct)-trnD(gtc)

Genome · 146943-148592 · 1650 bp

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Pi 0.197

MarkerSeek score 83.20

Primer available yes

Label trnS(gct)-trnD(gtc)

Feature Metadata

label_name: trnS(gct)-trnD(gtc)
feature_type: igs
region: Genome
length_bp: 1650
variable_sites: 294
pi: 0.197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gct)-trnD(gtc)_p1	GCAGCAGATGATGAAGGTCA	TGGGTGCATTAGGAGGAAGA	50.0	50.0	58.0	58.0	2767-2773 bp	1.0	91.5
trnS(gct)-trnD(gtc)_p2	TGCAGCAGATGATGAAGGTC	TGGGTGCATTAGGAGGAAGA	50.0	50.0	58.0	58.0	2768-2774 bp	1.0	91.5
trnS(gct)-trnD(gtc)_p3	GCAGCAGATGATGAAGGTCA	GGTGCATTAGGAGGAAGAGC	50.0	55.0	58.0	58.0	2765-2771 bp	1.0	91.4
trnS(gct)-trnD(gtc)_p4	GCAGCAGATGATGAAGGTCA	GTGCATTAGGAGGAAGAGCC	50.0	55.0	58.0	58.0	2770 bp	0.5	59.4
trnS(gct)-trnD(gtc)_p5	TGCAGCAGATGATGAAGGTC	GTGCATTAGGAGGAAGAGCC	50.0	55.0	58.0	58.0	2771 bp	0.5	59.4