Hotspot

trnL(tag)-trnN(gtt)

Genome · 107032-107200 · 169 bp

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Pi 0.149

MarkerSeek score 79.10

Primer available yes

Label trnL(tag)-trnN(gtt)

Feature Metadata

label_name: trnL(tag)-trnN(gtt)
feature_type: igs
region: Genome
length_bp: 169
variable_sites: 25
pi: 0.149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(tag)-trnN(gtt)_p1	AGCGTGTCTACCAATTCCAC	CCCCAGGAAGGATTTGAACC	50.0	55.0	57.9	58.1	268-274 bp	1.0	85.7
trnL(tag)-trnN(gtt)_p2	AGCGTGTCTACCAATTCCAC	AACCTCCCCAGGAAGGATTT	50.0	50.0	57.9	58.2	273-279 bp	1.0	85.0
trnL(tag)-trnN(gtt)_p3	CTACCAATTCCACCACGTCC	CCCCAGGAAGGATTTGAACC	55.0	55.0	58.3	58.1	261-267 bp	1.0	84.6
trnL(tag)-trnN(gtt)_p4	AATATGCGGACGGAGAGACT	CCCCAGGAAGGATTTGAACC	50.0	55.0	58.3	58.1	326-332 bp	1.0	82.7
trnL(tag)-trnN(gtt)_p5	ATATGCGGACGGAGAGACTT	CCCCAGGAAGGATTTGAACC	50.0	55.0	58.3	58.1	325-331 bp	1.0	82.7