Hotspot

psaF-psaJ

Genome · 92101-92124 · 24 bp

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Pi 0.13

MarkerSeek score 77.70

Primer available yes

Label psaF-psaJ

Feature Metadata

label_name: psaF-psaJ
feature_type: igs
region: Genome
length_bp: 24
variable_sites: 3
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF-psaJ_p1	GCAGGACTAACTCCATGCAA	AACGTTGCTTCCTTAAGGCA	50.0	45.0	57.9	58.0	1193-1194 bp	1.0	83.5
psaF-psaJ_p2	TTGCAGGACTAACTCCATGC	AACGTTGCTTCCTTAAGGCA	50.0	45.0	57.9	58.0	1195-1196 bp	1.0	83.5
psaF-psaJ_p3	GCAGGACTAACTCCATGCAA	CAGGTGCAATGTATTCGGGA	50.0	50.0	57.9	58.0	985-986 bp	1.0	83.3
psaF-psaJ_p4	CCATGCAAAGAATCAGCTGC	AACGTTGCTTCCTTAAGGCA	50.0	45.0	58.1	58.0	1182 bp	0.5	59.2
psaF-psaJ_p5	CCATGCAAAGAATCAGCTGC	CAGGTGCAATGTATTCGGGA	50.0	50.0	58.1	58.0	974 bp	0.5	59.1