Hotspot

ycf58-cemA

Genome · 44152-46519 · 2368 bp

Back to Mazzaella

Pi 0.106

MarkerSeek score 77.60

Primer available yes

Label ycf58-cemA

Feature Metadata

label_name: ycf58-cemA
feature_type: igs
region: Genome
length_bp: 2368
variable_sites: 251
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58-cemA_p1	TTGATGAGAGAGCCAGTTGC	ACCTCTGCATTTGGATCGAG	50.0	50.0	57.9	57.7	2550 bp	0.5	56.6
ycf58-cemA_p2	TTGATGAGAGAGCCAGTTGC	CCTCTGCATTTGGATCGAGT	50.0	50.0	57.9	57.7	2549 bp	0.5	56.6
ycf58-cemA_p3	GAGCCAGTTGCCTTCCATAT	ACCTCTGCATTTGGATCGAG	50.0	50.0	57.6	57.7	2541 bp	0.5	54.6
ycf58-cemA_p4	GAGCCAGTTGCCTTCCATAT	CCTCTGCATTTGGATCGAGT	50.0	50.0	57.6	57.7	2540 bp	0.5	54.6
ycf58-cemA_p5	AGAGCCAGTTGCCTTCCATA	CCTCTGCATTTGGATCGAGT	50.0	50.0	58.7	57.7	2541 bp	0.5	51.7