Hotspot

pdhB-petA

Genome · 10781-10844 · 64 bp

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Pi 0.121

MarkerSeek score 79.60

Primer available yes

Label pdhB-petA

Feature Metadata

label_name: pdhB-petA
feature_type: igs
region: Genome
length_bp: 64
variable_sites: 7
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pdhB-petA_p1	TCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	38.1	50.0	53.7	58.2	170-176 bp	1.0	46.0
pdhB-petA_p2	TCTGTGAGGAAATTAGTAGCT	TCTTTGCTGTGACACTTGCA	38.1	45.0	53.7	58.3	171-177 bp	1.0	46.0
pdhB-petA_p3	TTCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	36.4	50.0	54.6	58.2	171-177 bp	1.0	46.0
pdhB-petA_p4	AGATTCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	36.0	50.0	57.6	58.2	174-180 bp	1.0	45.9
pdhB-petA_p5	AGATTCTGTGAGGAAATTAGTAGCT	TCTTTGCTGTGACACTTGCA	36.0	45.0	57.6	58.3	175-181 bp	1.0	45.8