Hotspot

ycf1

SSC · 122405-128026 · 5622 bp

Pi 0.0135

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5622
variable_sites: 238
pi: 0.0135
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGAGTGTCCGGAAGAAGGA	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.1	58.0	820 bp	1.0	83.9
ycf1_p2	ATGAGTGTCCGGAAGAAGGA	GCAGAATACCGTCACCCATT	50.0	50.0	58.1	58.0	950 bp	1.0	83.5
ycf1_p3	GTAGGCGGGGATATCCTTCT	ACAGGAACAAGAGGGATCCA	55.0	50.0	58.1	58.0	486 bp	0.888889	79.0
ycf1_p4	GTAGGCGGGGATATCCTTCT	AAGAGGGATCCACCGAAGAA	55.0	50.0	58.1	58.0	478 bp	0.888889	79.0
ycf1_p5	GTAGGCGGGGATATCCTTCT	GCAGAATACCGTCACCCATT	55.0	50.0	58.1	58.0	616 bp	0.888889	78.3