Hotspot

rps15-ycf1

SSC · 121982-122404 · 423 bp

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Pi 0.028
MarkerSeek score 57.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
423
variable_sites
36
pi
0.028
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT AACCCATCCGTATGTTTGCA 45.0 45.0 58.1 57.8 1384-1408 bp 1.0 83.3
rps15-ycf1_p2 AACACCATGCATTGATGGGT ACCCATCCGTATGTTTGCAA 45.0 45.0 58.1 57.8 1383-1407 bp 1.0 83.3
rps15-ycf1_p3 AGCTGTTCGGGACCATTTAC ACCCATCCGTATGTTTGCAA 50.0 45.0 57.9 57.8 1574-1598 bp 1.0 82.5
rps15-ycf1_p4 GCGTTGACGTTTTCCCAAAA AACCCATCCGTATGTTTGCA 45.0 45.0 58.1 57.8 1126-1150 bp 0.888889 76.6
rps15-ycf1_p5 GCGTTGACGTTTTCCCAAAA ACCCATCCGTATGTTTGCAA 45.0 45.0 58.1 57.8 1125-1149 bp 0.888889 76.6