Hotspot

ndhD-psaC

SSC · 115738-115850 · 113 bp

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Pi 0.0331
MarkerSeek score 61.80
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
113
variable_sites
13
pi
0.0331
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TCGTGGTAAAGACAAGATACAC GACCGAAGACTGTGTTGGTT 40.9 50.0 55.9 58.1 239-252 bp 1.0 54.4
ndhD-psaC_p2 TCGTGGTAAAGACAAGATACAC AAGAGATGTGAATCCGCCTG 40.9 50.0 55.9 57.7 217-230 bp 1.0 53.0
ndhD-psaC_p3 TCGTGGTAAAGACAAGATACAC AAGGACCGAAGACTGTGTTG 40.9 50.0 55.9 57.8 242-255 bp 1.0 52.8
ndhD-psaC_p4 TCGTGGTAAAGACAAGATACAC CAAGGACCGAAGACTGTGTT 40.9 50.0 55.9 57.8 243-256 bp 1.0 52.7
ndhD-psaC_p5 TCGTGGTAAAGACAAGATACAC CCGAAGACTGTGTTGGTTGT 40.9 50.0 55.9 58.3 237-250 bp 1.0 52.2