Hotspot

psbE-petL

LSC · 64769-65644 · 876 bp

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Pi 0.018
MarkerSeek score 54.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
876
variable_sites
49
pi
0.018
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATGGAATTCCTTGTCGGCTC CACCGAATCTCGCAGAAGAA 50.0 50.0 58.0 58.0 1211-1222 bp 1.0 85.2
psbE-petL_p2 CAACCCGCAATGAATAGGGA AAGCGCTCTTAGTTCAGCTC 50.0 50.0 57.9 58.0 1551-1564 bp 1.0 84.6
psbE-petL_p3 ATGGAATTCCTTGTCGGCTC AAGCGCTCTTAGTTCAGCTC 50.0 50.0 58.0 58.0 1640-1653 bp 1.0 84.5
psbE-petL_p4 TCGTTCGGGTTTGATTGTGT CACCGAATCTCGCAGAAGAA 45.0 50.0 58.0 58.0 1450-1461 bp 1.0 84.3
psbE-petL_p5 TCGTTCGGGTTTGATTGTGT AAGCGCTCTTAGTTCAGCTC 45.0 50.0 58.0 58.0 1879-1892 bp 1.0 83.9