Hotspot

petA-psbJ

LSC · 63003-63981 · 979 bp

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Pi 0.0158
MarkerSeek score 50.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
979
variable_sites
52
pi
0.0158
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TTTATCCCGACGGAAGCAAG ACACAATCAAACCCGAACGA 50.0 45.0 58.0 58.0 1730-1754 bp 1.0 83.6
petA-psbJ_p2 TCCGATCGTATTTCTCCCGA ACACAATCAAACCCGAACGA 50.0 45.0 58.0 58.0 1950-1974 bp 1.0 83.2
petA-psbJ_p3 TTTATCCCGACGGAAGCAAG GAGCCGACAAGGAATTCCAT 50.0 50.0 58.0 58.0 1969-1993 bp 1.0 83.1
petA-psbJ_p4 TTTATCCCGACGGAAGCAAG TCCCTATTCATTGCGGGTTG 50.0 50.0 58.0 57.9 2058-2082 bp 1.0 82.9
petA-psbJ_p5 TCCGATCGTATTTCTCCCGA GAGCCGACAAGGAATTCCAT 50.0 50.0 58.0 58.0 2189-2213 bp 1.0 82.8