Hotspot

psaA-ycf3

LSC · 41164-41884 · 721 bp

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Pi 0.0176
MarkerSeek score 57.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
721
variable_sites
40
pi
0.0176
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TTTTACTTCTGGTTCCGGCG ATTGGTTGAAGATCACCCGG 50.0 50.0 58.5 57.9 781-812 bp 1.0 81.0
psaA-ycf3_p2 TTTACTTCTGGTTCCGGCG ATTGGTTGAAGATCACCCGG 52.6 50.0 57.8 57.9 780-811 bp 1.0 75.1
psaA-ycf3_p3 TTACTTCTGGTTCCGGCGAA ATTGGTTGAAGATCACCCGG 50.0 50.0 59.3 57.9 779-810 bp 1.0 74.3
psaA-ycf3_p4 TTTACTTCTGGTTCCGGCGA ATTGGTTGAAGATCACCCGG 50.0 50.0 59.3 57.9 780-811 bp 1.0 74.3
psaA-ycf3_p5 TACTTCTGGTTCCGGCGAA ATTGGTTGAAGATCACCCGG 52.6 50.0 58.6 57.9 778-809 bp 1.0 71.7