Hotspot

trnT-GGU-psbD

LSC · 31294-32216 · 923 bp

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Pi 0.0135
MarkerSeek score 50.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
923
variable_sites
36
pi
0.0135
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1049-1397 bp 1.0 80.9
trnT-GGU-psbD_p2 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.3 58.2 1039-1387 bp 1.0 79.9
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CAAAAACGAAACGGTCCCTC 50.0 50.0 58.3 57.6 1038-1386 bp 1.0 78.7
trnT-GGU-psbD_p4 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1024-1372 bp 1.0 77.8
trnT-GGU-psbD_p5 GCCATGGTAAGGCGTAAGTC GGACCAGCCTACAAAAACGA 55.0 50.0 58.7 58.1 1059-1407 bp 1.0 77.5