Hotspot

rpoB-trnC-GCA

LSC · 27122-28353 · 1232 bp

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Pi 0.0101
MarkerSeek score 55.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1232
variable_sites
38
pi
0.0101
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTGCCCCATTTGCATCCA CCGGATTTGAACTGGGGAAA 47.4 50.0 58.0 58.1 1280-1320 bp 1.0 77.2
rpoB-trnC-GCA_p2 ATTGCCCCATTTGCATCCA TTGTTGATTAGGCGACACCC 47.4 50.0 58.0 58.2 1298-1338 bp 1.0 76.3
rpoB-trnC-GCA_p3 CCATTTGCATCCACGAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.3 58.1 1274-1314 bp 1.0 75.2
rpoB-trnC-GCA_p4 ATTGCCCCATTTGCATCCA AAAAGGATTTGCAGTCCCCC 47.4 50.0 58.0 58.4 1262-1302 bp 1.0 75.1
rpoB-trnC-GCA_p5 CCATTTGCATCCACGAGCAT TTGTTGATTAGGCGACACCC 50.0 50.0 59.3 58.2 1292-1332 bp 1.0 74.3