Hotspot

rps2-rpoC2

LSC · 16575-16741 · 167 bp

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Pi 0.0236
MarkerSeek score 55.80
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
167
variable_sites
11
pi
0.0236
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCTCCAGCATCTCTTCCAAG TGCATGATAGACTAGAACAATCA 55.0 34.8 57.7 55.2 260-322 bp 1.0 44.1
rps2-rpoC2_p2 CCTCCAGCATCTCTTCCAAG TTGCATGATAGACTAGAACAATCA 55.0 33.3 57.7 55.9 261-323 bp 1.0 44.1
rps2-rpoC2_p3 AAATGAACTCCTGCCTCCAG TGCATGATAGACTAGAACAATCA 50.0 34.8 57.5 55.2 273-335 bp 1.0 43.9
rps2-rpoC2_p4 GCCAAAATGAACTCCTGCCT TGCATGATAGACTAGAACAATCA 50.0 34.8 58.7 55.2 277-339 bp 1.0 43.9
rps2-rpoC2_p5 CCATGGCCAAAATGAACTCC TGCATGATAGACTAGAACAATCA 50.0 34.8 57.3 55.2 282-344 bp 1.0 43.8