Hotspot

trnS-GCU-trnG-UCC

LSC · 8447-9221 · 775 bp

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Pi 0.0176
MarkerSeek score 60.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
775
variable_sites
40
pi
0.0176
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AGGGTTAATCGAACGAATCACA 50.0 40.9 58.0 57.8 834-876 bp 1.0 70.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AGGGTTAATCGAACGAATCACA 50.0 40.9 58.0 57.8 833-875 bp 1.0 70.1
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GGGTTAATCGAACGAATCACAC 50.0 45.5 58.0 57.7 833-875 bp 1.0 69.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGGTTAATCGAACGAATCACAC 50.0 45.5 58.0 57.7 832-874 bp 1.0 69.7
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA AGGGTTAATCGAACGAATCACA 50.0 40.9 58.2 57.8 888-930 bp 1.0 68.7