Hotspot

rps16-trnQ-UUG

LSC · 6085-7197 · 1113 bp

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Pi 0.0167
MarkerSeek score 56.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1113
variable_sites
57
pi
0.0167
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GGTTCGAATCCTTCCGTCC 50.0 57.9 57.9 57.9 1153-1202 bp 1.0 78.7
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG GTTCGAATCCTTCCGTCCC 50.0 57.9 57.9 57.9 1152-1201 bp 1.0 78.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGTTCGAATCCTTCCGTCC 45.0 57.9 58.1 57.9 1136-1185 bp 1.0 78.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 1135-1184 bp 1.0 78.3
rps16-trnQ-UUG_p5 CAACGGATCGTGTCCTTCAA GTTCGAATCCTTCCGTCCC 50.0 57.9 58.2 57.9 1153-1202 bp 1.0 77.7