Hotspot

trnK-UUU-rps16

LSC · 4223-4941 · 719 bp

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Pi 0.0268
MarkerSeek score 70.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
719
variable_sites
59
pi
0.0268
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGGATCAGTCGTGGTCTT GGGTTTGTTGCTGCCATTTT 50.0 45.0 58.1 58.0 1544-1571 bp 0.888889 79.7
trnK-UUU-rps16_p2 TTCAGGATCAGTCGTGGTCT GGGTTTGTTGCTGCCATTTT 50.0 45.0 58.1 58.0 1545-1572 bp 0.888889 79.7
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT AAAAAGGGTTTGTTGCTGCC 50.0 45.0 58.1 58.0 1549-1576 bp 0.888889 79.7
trnK-UUU-rps16_p4 TTCAGGATCAGTCGTGGTCT AAAAAGGGTTTGTTGCTGCC 50.0 45.0 58.1 58.0 1550-1577 bp 0.888889 79.7
trnK-UUU-rps16_p5 TCAGGATCAGTCGTGGTCTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.1 57.9 1132-1151 bp 0.888889 79.7