Hotspot

petD

LSC · 78805-80068 · 1264 bp

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Pi 0.00316

MarkerSeek score 60.90

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1264
variable_sites: 6
pi: 0.00316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGACATGACTAGTTGACGGG	GCACATTCCAGATATTTTGAA	50.0	33.3	57.0	52.5	1428-1429 bp	1.0	40.4
petD_p2	TTGACATGACTAGTTGACGGG	GCACATTCCAGATATTTTGAA	47.6	33.3	57.7	52.5	1429-1430 bp	1.0	40.4
petD_p3	GACATGACTAGTTGACGGGA	GCACATTCCAGATATTTTGAA	50.0	33.3	56.7	52.5	1427-1428 bp	1.0	40.4
petD_p4	TGACATGACTAGTTGACGGG	GCACATTCCAGATATTTTGAAT	50.0	31.8	57.0	53.1	1428-1429 bp	1.0	40.4
petD_p5	TGACATGACTAGTTGACGGG	TGGAAATGGAAAATTCACTTT	50.0	28.6	57.0	52.0	1374-1375 bp	1.0	40.4