Hotspot

ndhF

SSC · 114183-115673 · 1491 bp

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Pi 0.00224

MarkerSeek score 60.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 1491
variable_sites: 5
pi: 0.00224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAAAGGCCCTCGTTATCTT	AAAAGGGACAAGAACGAGGG	50.0	50.0	57.7	57.7	1635 bp	1.0	75.6
ndhF_p2	AGTACGAAAGGCCCTCGTTA	AAAAGGGACAAGAACGAGGG	50.0	50.0	58.5	57.7	1639 bp	1.0	74.5
ndhF_p3	TAGTACGAAAGGCCCTCGTT	AAAAGGGACAAGAACGAGGG	50.0	50.0	58.5	57.7	1640 bp	1.0	74.5
ndhF_p4	GTACGAAAGGCCCTCGTTAT	AAAAGGGACAAGAACGAGGG	50.0	50.0	57.4	57.7	1638 bp	1.0	73.3
ndhF_p5	CGAAAGGCCCTCGTTATCTT	AAAGGGACAAGAACGAGGGA	50.0	50.0	57.7	58.6	1634 bp	1.0	73.2