Hotspot

rpl20-rps12

LSC · 71065-71829 · 765 bp

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Pi 0.00261

MarkerSeek score 47.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 3
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGTTCTACGGTTCCGAGCTA	CCGAGGAACATGTATTCGGG	50.0	55.0	58.2	58.1	835 bp	1.0	77.8
rpl20-rps12_p2	TTTGTTCTACGGTTCCGAGC	CCGAGGAACATGTATTCGGG	50.0	55.0	58.2	58.1	837 bp	1.0	77.5
rpl20-rps12_p3	TGTTCTACGGTTCCGAGCTA	GCTCTTAGGGGATGTCCTCA	50.0	55.0	58.2	58.2	858 bp	1.0	77.2
rpl20-rps12_p4	TGTTCTACGGTTCCGAGCTA	TCTTAGGGGATGTCCTCAGC	50.0	55.0	58.2	58.2	856 bp	1.0	77.2
rpl20-rps12_p5	GTAAGTCTTGAACGAGCCCC	CCGAGGAACATGTATTCGGG	55.0	55.0	58.3	58.1	885 bp	1.0	77.0