Hotspot

psbE-petL

LSC · 66621-67754 · 1134 bp

Back to Maytenus

Pi 0.00412

MarkerSeek score 61.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 7
pi: 0.00412
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.9	57.0	1279-1282 bp	1.0	72.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.8	57.0	1202-1205 bp	1.0	71.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCCGCTAATAGAAAACCGA	45.0	45.0	58.2	57.0	1288-1291 bp	1.0	71.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCCGCTAATAGAAAACCGA	50.0	45.0	58.2	57.0	1293-1296 bp	1.0	71.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.8	57.0	1287-1290 bp	1.0	71.2