Hotspot

atpB-rbcL

LSC · 56692-57443 · 752 bp

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Pi 0.00177

MarkerSeek score 59.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 2
pi: 0.00177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGAGCAATACGTCCGAGG	CCAGCCTTGAATCCAACACT	50.0	50.0	58.0	58.1	875-876 bp	1.0	79.5
atpB-rbcL_p2	GAGCAATACGTCCGAGGTTT	CCAGCCTTGAATCCAACACT	50.0	50.0	58.0	58.1	872-873 bp	1.0	79.5
atpB-rbcL_p3	TTTGAGCAATACGTCCGAGG	GTCTCGGTTTGTGGTGACAT	50.0	50.0	58.0	58.1	848-849 bp	1.0	79.2
atpB-rbcL_p4	ACCTCCATGCCAGAAGTAGT	CCAGCCTTGAATCCAACACT	50.0	50.0	58.0	58.1	834-835 bp	1.0	79.2
atpB-rbcL_p5	GAGCAATACGTCCGAGGTTT	GTCTCGGTTTGTGGTGACAT	50.0	50.0	58.0	58.1	845-846 bp	1.0	79.2