Hotspot

psbZ-trnG-GCC

LSC · 37171-37861 · 691 bp

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Pi 0.00482

MarkerSeek score 63.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 5
pi: 0.00482
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	843-849 bp	1.0	77.6
psbZ-trnG-GCC_p2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	832-838 bp	1.0	77.4
psbZ-trnG-GCC_p3	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	833-839 bp	1.0	76.9
psbZ-trnG-GCC_p4	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	834-840 bp	1.0	73.9
psbZ-trnG-GCC_p5	GAGGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	831-837 bp	1.0	73.2