Hotspot

ycf1

SSC · 123435-128807 · 5373 bp

Back to Matthiola

Pi 0.0409

MarkerSeek score 67.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5373
variable_sites: 435
pi: 0.0409
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTCCGCGGGTTTATTTTGA	CGTTCCCAAAGGCGTAAAAC	45.0	50.0	57.8	58.0	964-973 bp	0.8	77.4
ycf1_p2	TGTCTTTTGAATGTCCGCCA	CGTTCCCAAAGGCGTAAAAC	45.0	50.0	58.0	58.0	1148-1157 bp	0.6	67.4
ycf1_p3	AACCATAACGCGGGTTGTTA	CGTTCCCAAAGGCGTAAAAC	45.0	50.0	57.8	58.0	1099-1108 bp	0.6	66.2
ycf1_p4	TAACCATAACGCGGGTTGTT	CGTTCCCAAAGGCGTAAAAC	45.0	50.0	57.8	58.0	1100-1109 bp	0.6	66.2
ycf1_p5	TGTCTTTTGAATGTCCGCCA	TTCAAATTCCCGAGTGGTCC	45.0	50.0	58.0	57.8	793-799 bp	0.4	56.5