Hotspot

ndhG-ndhI

SSC · 118395-118789 · 395 bp

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Pi 0.106

MarkerSeek score 76.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 71
pi: 0.106
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	987-1048 bp	1.0	89.5
ndhG-ndhI_p2	GTGCAGCAGCTACAAAATGG	GTCAACAAACCCTACGAGCT	50.0	50.0	58.0	57.8	988-1049 bp	1.0	89.5
ndhG-ndhI_p3	GCCCCCACGTAAATAAGGAG	TCAACAAACCCTACGAGCTG	55.0	50.0	58.0	57.8	1009-1070 bp	1.0	89.2
ndhG-ndhI_p4	GCCCCCACGTAAATAAGGAG	GTCAACAAACCCTACGAGCT	55.0	50.0	58.0	57.8	1010-1071 bp	1.0	89.2
ndhG-ndhI_p5	TCCGTACCATGACGTATCGA	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	1185-1230 bp	0.6	68.9